MolPharm

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FRANKLIN, T. J.
Right arrow Articles by TWOSE, P. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by FRANKLIN, T. J.
Right arrow Articles by TWOSE, P. A.

Molecular Pharmacology, Vol 11, 485-491, Copyright © 1975 by the American Society for Pharmacology and Experimental Therapeutics

Desensitization of Beta Adrenergic Receptors in Human Fibroblasts in Tissue Culture

T. J. FRANKLIN 1, W. P. MORRIS 1, and P. A. TWOSE 1

1 Department of Biochemistry, Pharmaceuticals Division, Imperial Chemical Industries, Ltd., Alderley Park, Macclesfield, Cheshire, England

The ability of isoproterenol, epinephrine, norepinephrine, and salbutamol to desensitize the beta receptor-coupled cyclic 3',5'-AMP response of human diploid fibroblasts in tissue culture correlates well with their activity as beta stimulants. Partial desensitization of the cells results in a marked fall in the maximal cyclic AMP response to isoproterenol: fully desensitized cells are totally unresponsive to isoproterenol. Recovery of beta adrenergic sensitivity is a slow process and is delayed by extremely low concentrations of isoproterenol (picomolar). Recovery is also inhibited by concentrations of puromycin, cycloheximide, and actinomycin D that inhibit protein or RNA synthesis.

Submitted on February 5, 1975






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1975 by the American Society for Pharmacology and Experimental Therapeutics