Molecular Pharmacology, Vol 2, 266-268, Copyright © 1966 by the American Society for Pharmacology and Experimental Therapeutics

Drug Action and Aminoacyl RNA Ligases

¹ Microbiology Department, University of Auckland, Auckland, New Zealand

The effects of insulin, testosterone, hydrocortisone, dibenz[a,j]acridine, stilbestrol, 2-ethylisothionicotinamide, tetracycline, D-4-amino-3-isoxazolidone, streptomycin, and Pactamycin on activation and transfer of glycine, valine, phenylalanine, histidine, methionine, glutamic acid, and lysine were tested in an in vitro system from calf liver.

Aminoacylation by phenylalanine, histidine, methionine, and glutamic acid was greatly reduced in the presence of 2-ethylisothionicotinamide.

Note:
We wish to thank May and Baker (N.Z.) Ltd. for the gift of 2-ethylisothionicotinamide and the Upjohn Co. (U.S.A.) for the sample of Paetamycin. We thank Drs. P. L. Bergquist and B. C. Baguley for their assistance with these studies and the Westfield Freezing Co. Ltd. for cooperation in supplying fresh tissue. This work was supported in part by grants from time U.S. Public Health Service (AI-04973-01) and the N.Z. Cancer Society, Auckland Branch.