MolPharm

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jekunen, A.
Right arrow Articles by Vilpo, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jekunen, A.
Right arrow Articles by Vilpo, J. A.

5-Methyl-2'-deoxycytidine. Metabolism and effects on cell lethality studied with human leukemic cells in vitro

A Jekunen and JA Vilpo

5-Methylcytosine ( 5MeCyt ) is a possible regulator of eukaryotic gene transcription. We investigated whether this compound could be introduced into DNA from exogenous deoxyribonucleoside 5-methyl-2'- deoxycytidine ( 5MedCyd ). High concentrations of 5MedCyd inhibited the growth of several types of human leukemic cell lines in vitro. However, the effect could be accounted for by dThd, a deamination product of 5MedCyd . We found that radioactivity from [methyl-14C] 5MedCyd and [2- 14C] 5MedCyd was incorporated into DNA as thymidylate, and none was present as 5MeCyt . There are two conceivable metabolic pathways from 5MedCyd to thymidylate. The first consists of deoxycytidine or thymidine kinase and deoxycytidylate deaminase, and the second of sequential reactions catalyzed by deoxycytidine deaminase and thymidine kinase. No indication of the first pathway was demonstrable in human leukemic cells. We conclude that the DNA exclusion of 5MeCyt from exogenous 5MedCyd takes place because of powerful deoxycytidine deaminase activity in human malignant hematopoietic cells.

Volume 25, Issue 3, pp. 431-435, 05/01/1984
Copyright © 1984 by American Society for Pharmacology and Experimental Therapeutics






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1984 by the American Society for Pharmacology and Experimental Therapeutics