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Evidence That Curcumin Suppresses the Growth of Malignant Gliomas in Vitro and in Vivo through Induction of Autophagy: Role of Akt and Extracellular Signal-Regulated Kinase Signaling Pathways

Departments of Neurosurgery (H.A., S.K., R.S., Y.K.) and Experimental Therapeutics (Y.T., B.B.A.), the University of Texas M. D. Anderson Cancer Center, Houston, Texas; Department of Neurosurgery, the Baylor College of Medicine, Houston, Texas (S.K., R.S.); and the Program in Molecular Pathology, the University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas (S.K.)

Received December 1, 2006; accepted March 28, 2007

	Abstract

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Autophagy is a response of cancer cells to various anticancer therapies. It is designated as programmed cell death type II and characterized by the formation of autophagic vacuoles in the cytoplasm. The Akt/mammalian target of rapamycin (mTOR)/p70 ribosomal protein S6 kinase (p70S6K) and the extracellular signal-regulated kinases 1/2 (ERK1/2) pathways are two major pathways that regulate autophagy induced by nutrient starvation. These pathways are also frequently associated with oncogenesis in a variety of cancer cell types, including malignant gliomas. However, few studies have examined both of these signal pathways in the context of anticancer therapy-induced autophagy in cancer cells, and the effect of autophagy on cell death remains unclear. Here, we examined the anticancer efficacy and mechanisms of curcumin, a natural compound with low toxicity in normal cells, in U87-MG and U373-MG malignant glioma cells. Curcumin induced G₂/M arrest and nonapoptotic autophagic cell death in both cell types. It inhibited the Akt/mTOR/p70S6K pathway and activated the ERK1/2 pathway, resulting in induction of autophagy. It is interesting that activation of the Akt pathway inhibited curcumin-induced autophagy and cytotoxicity, whereas inhibition of the ERK1/2 pathway inhibited curcumin-induced autophagy and induced apoptosis, thus resulting in enhanced cytotoxicity. These results imply that the effect of autophagy on cell death may be pathway-specific. In the subcutaneous xenograft model of U87-MG cells, curcumin inhibited tumor growth significantly (P < 0.05) and induced autophagy. These results suggest that curcumin has high anticancer efficacy in vitro and in vivo by inducing autophagy and warrant further investigation toward possible clinical application in patients with malignant glioma.

Accumulating evidence shows that a natural product, curcumin (diferuloylmethane), has a potent anticancer effect both in vitro and in vivo on a variety of cancer cell types, such as leukemia, breast cancer, prostate cancer, and pancreatic cancer (Aggarwal et al., 2003; Shishodia et al., 2005). However, the efficacy of curcumin for malignant glioma cells in vitro and in vivo is not yet fully determined. As expected from the fact that curcumin is an active ingredient of the spice turmeric, it caused no serious toxicity in animal studies (up to 5 g/kg; Wahlstrom and Blennow, 1978), and it was safely administered to humans without major toxicity in phase I clinical studies (up to 12 g/day; Sharma et al., 2001, 2004; Lao et al., 2006). However, these findings also underscore that we need to overcome the low absorption and bioavailability of curcumin outside of the colon to use it as systemic cancer preventive agent.

Several mechanisms by which curcumin exerts its anticancer effect have been reported. First, curcumin inhibits a transcription factor, nuclear factor B (NF-B), by inhibiting inhibitor of B kinase and subsequent IB phosphorylation (Singh and Aggarwal, 1995; Bharti et al., 2003; Aggarwal et al., 2004, 2006). As a result, curcumin down-regulates the expression of NF-B-regulated gene products such as Bcl-2, Bcl-X_L, cyclin D1, matrix metalloproteinase-9, cyclooxygenase-2, and interleukin-6, resulting in cell cycle arrest, suppression of proliferation, and induction of apoptosis (Mukhopadhyay et al., 2002; Bharti et al., 2003; Aggarwal et al., 2004, 2006). Second, curcumin inhibits the Akt/mammalian target of rapamycin (mTOR) pathway and phosphorylation of p70 ribosomal protein S6 kinase (p70S6K) and eukaryotic initiation factor 4E-binding protein, resulting in inhibition of proliferation and induction of apoptosis (Woo et al., 2003; Bava et al., 2005; Aggarwal et al., 2006; Beevers et al., 2006). Other mechanisms of the antitumor effect of curcumin include down-regulation of transcription factors activator protein-1 (Nakamura et al., 2002; Prusty and Das, 2005; Tomita et al., 2006) and Egr-1 (Chen et al., 2006).

Autophagy has attracted the interest of scientists in the field of cancer research because it is designated as programmed cell death type II, whereas apoptosis is wellknown as programmed cell death type I (Bursch et al., 2000). Autophagic cell death is characterized by numerous autophagic vacuoles in the cytoplasm, whereas the nucleus remains intact until the late stage of cell death. In contrast, apoptosis is manifested by DNA condensation and fragmentation. We have reported that malignant glioma cells are very resistant to apoptosis but that they undergo autophagy in response to anticancer therapies such as radiation, temozolomide, and ceramide (Daido et al., 2004, 2005; Kanzawa et al., 2004; Ito et al., 2005). Autophagy is basically a protein degradation system of the cell's own lysosomes (Klionsky and Emr, 2000). It is a process that maintains ATP level and is typically activated on amino acid deprivation (Meijer and Codogno, 2004; Kelekar, 2006). On the other hand, amino acids and ATP are negative regulators of autophagy. As a sensor of amino acids and ATP, mTOR negatively regulates autophagy through the mTOR/p70S6K pathway by activating this pathway in response to amino acids and ATP (Blommaart et al., 1995; Shigemitsu et al., 1999). Furthermore, PTEN and Akt are upstream regulators of the mTOR pathway: PTEN induces autophagy, and Akt inhibits autophagy (Arico et al., 2001). The Raf-1/MEK1/2/ERK1/2 pathway is another pathway that mediates signals stimulated by amino acids: amino acids inhibit this pathway and autophagy. ERK phosphorylates G-interacting protein, which accelerates the rate of GTP hydrolysis by the G_i3 protein, resulting in induction of autophagy (Ogier-Denis et al., 2000; Pattingre et al., 2003). Although it is established that the Akt/mTOR/p70S6K pathway and the Raf-1/MEK1/2/ERK1/2 pathway are involved in regulating autophagy, their roles in autophagy in cancer are not yet fully determined.

In the present study, we investigated the anticancer effect of curcumin on U87-MG and U373-MG human malignant glioma cells in vitro and in vivo. We found that curcumin efficiently inhibited growth of these cell types by inducing nonapoptotic autophagic cell death. Furthermore, we examined the signal pathways of curcumin-induced autophagy and investigated the role of the pathways in cell death. To the best of our knowledge, this is the first study to demonstrate that curcumin induces autophagy, which is regulated by simultaneous inhibition of the Akt/mTOR/p70S6K pathway and stimulation of the ERK1/2 pathway.

	Materials and Methods

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Reagents. Curcumin with a purity greater than 95% was kindly supplied by Sabinsa Corporation (Piscataway, NJ) and dissolved in DMSO (Sigma, St. Louis, MO) to produce a 100 mM stock solution. Acridine orange was purchased from Polysciences (Warrington, PA). 3-Methyladenine (3-MA), a phosphatidylinositol 3-phosphate kinase (PI3K) inhibitor, was purchased from Sigma. PD98059, a mitogenactivated protein kinase/extracellular signal-regulated kinase kinase 1 (MEK1) inhibitor, was purchased from Cell Signaling Technology (Danvers, MA). Paclitaxel (Taxol) was purchased from Bristol-Myers Squibb (Princeton, NJ). A recombinant full-length human active Akt1 protein (rAkt1) was purchased from Upstate (Temecula, CA).

Cell Culture. U87-MG and U373-MG human malignant glioma cells with PTEN mutation and KBM-5 human leukemia cells were purchased from the American Type Culture Collection (Manassas, VA). Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10 to 15% fetal bovine serum, 100 U/ml penicillin, and 2.5 µg/ml antimycotic (Fungizone; all from Invitrogen, Carlsbad, CA) at 37°C in 5% CO₂.

Cell Viability Assay. The cytotoxic effect of curcumin was determined by using the cell proliferation reagent WST-1 (Roche Applied Science, Indianapolis, IN), as described previously (Ito et al., 2006). In brief, U87-MG and U373-MG cells were seeded at 3 x 10³ cells/well in 96-well flat-bottomed plates and incubated at 37°C overnight. After cells were treated with 0, 10, 30, 50, 70, or 90 µM curcumin for 72 h, they were exposed to 10 µl of the WST-1 reagent for 1 h at 37°C. The absorbance at 450 nm was measured using a microplate reader. The viability of untreated cells was considered to be 100%.

Cell Cycle Analysis. Tumor cells treated with curcumin (0, 20, and 40 µM) for 72 h were trypsinized, fixed with ice-cold 70% ethanol, stained with propidium iodide by using a cellular DNA flow cytometric analysis reagent set (Roche), and analyzed for DNA content by FACScan (Becton Dickinson, San Jose, CA). Data were analyzed by Cell Quest software (Becton Dickinson). At least 100,000 cells were analyzed for each sample. Paclitaxel (5 nM) was used as a positive control to induce apoptosis (Kondo and Kondo, 2006).

Apoptosis Detection Assay. Tumor cells were seeded on Lab-Tek chamber slides (Nunc, Rochester, NY) and incubated overnight and then were treated with 40 µM curcumin for 72 h and stained with the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) technique using an ApopTag apoptosis detection kit (Chemicon, Temecula, CA) as described previously (Takeuchi et al., 2005). Two hundred cells were counted and scored for the incidence of positive staining under a microscope. Paclitaxel (5 nM) was used as a positive control to induce apoptosis.

Clonogenic Assay. Tumor cells were diluted serially and seeded into the six-well plates in triplicate per data point. Twenty-four hours after seeding, cells were treated with different concentrations of curcumin as indicated. Two weeks after treatment, cells were fixed and stained with 0.5% crystal violet (Sigma) in methanol for 5 min. Then, colonies consisting of 50 or more cells were counted.

Electron Microscopy. To detect the induction of autophagy morphologically in curcumin-treated tumor cells, we performed ultrastructural analysis. Cells were grown on glass coverslips, treated with 40 µM curcumin for 48 h, and then fixed with a solution containing 3% glutaraldehyde plus 2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.3, for 1 h. After fixation, the samples were postfixed in 1% OsO₄ in the same buffer for 1 h and then subjected to electron microscopic analysis. Representative areas were chosen for ultrathin sectioning and viewed with a JEM 1010 transmission electron microscope (JEOL, Peabody, MA) at an accelerating voltage of 80 kV. Digital images were obtained with an AMT imaging system (Advanced Microscopy Techniques, Danvers, MA).

Detection and Quantification of Acidic Vesicular Organelles with Acridine Orange Staining. Autophagy is the process of sequestering cytoplasmic proteins into the lytic component and is characterized by the formation and promotion of acidic vesicular organelles (AVOs) as described previously (Paglin et al., 2001). To detect the development of AVOs, we treated cells with 20 and 40 µM curcumin for 72 h and then performed vital staining with acridine orange. To quantify the development of AVOs, the cells were stained with acridine orange (1 µg/ml) for 15 min, removed from the plate with trypsin-EDTA (Invitrogen), and analyzed using a FACScan flow cytometer and CellQuest software. To inhibit autophagy, 2.0 mM 3-MA was added 24 h after the addition of curcumin.

GFP-LC3 Dot Assay. The green fluorescent protein (GFP)-tagged microtubule-associated protein 1 light chain 3 (LC3) expression vector was kindly provided by Dr. Noboru Mizushima (Tokyo Medical and Dental University, Tokyo, Japan). LC3 is recruited to the autophagosomal membrane during autophagy (Kabeya et al., 2000). Therefore, GFP-tagged LC3-expressing cells have been used to demonstrate the induction of autophagy (Kabeya et al., 2000; Kanzawa et al., 2004; Mizushima, 2004). GFP-LC3 cells present a diffuse distribution under control conditions, whereas a punctate pattern of GFP-LC3 expression (GFP-LC3 dots) is induced by autophagy. Cells were transiently transfected with the GFP-LC3 vector using Fugene 6 transfection reagent (Roche). After overnight culture, cells were treated with 20 and 40 µM curcumin for 72 h, fixed with 4% paraformaldehyde, and examined under a fluorescence microscope. To quantify autophagic cells after curcumin treatment, we counted the number of autophagic cells demonstrating GFP-LC3 dots (10 dots/cell) among 200 GFP-positive cells.

Western Blotting. Soluble proteins were isolated from untreated and curcumin-treated cells. For the detection of LC3, poly(ADP-ribose) polymerase (PARP), and NF-B p65, culture medium with 10% fetal bovine serum was used. For the detection of signal pathway molecules phospho-Akt, phospho-p70S6K, and phospho-ERK, culture medium with low serum (0.5% fetal bovine serum) was used for up to 24 h to exclude the effects of growth factors contained in the serum. Equal amounts of protein were separated by 10 or 15% SDS-polyacrylamide gel electrophoresis gel (Bio-Rad Laboratories, Richmond, CA) and transferred to a Hybond-P membrane (GE Healthcare, Chalfont St. Giles, Buckinghamshire, UK). The membranes were treated with primary antibodies overnight at 4°C and incubated for 1 h with a horseradish peroxidase-conjugated antimouse or anti-rabbit secondary antibody (1:3000 dilution; GE Healthcare) at room temperature for 1 h. Bound antibody complexes were detected using an enhanced chemiluminescence reagent (GE Healthcare) according to the manufacturer's instructions. Anti-LC3 antibody against a synthetic peptide corresponding to the N-terminal 14 amino acids of isoform B LC3 and an additional cysteine (PSEKTFKQRRTFEQC) was prepared by immunization of rabbit and was affinity-purified on an immobilized peptide-Sepharose column (Covance Research Products, Princeton, NJ). We purchased anti--actin (Sigma), anti-phospho-Akt at Ser⁴⁷³, anti-total Akt, anti-phospho-p70S6K at Thr³⁸⁹, anti-total p70S6K, anti-phospho-ERK1/2 at Thr²⁰²/Tyr²⁰⁴, anti-total ERK1/2, anti-phospho-PP1 at Thr³²⁰, anti-total PP1, and anti-PARP antibodies from Cell Signaling Technology. Anti-phospho-PP2A at Thr³⁰⁷ and anti-total PP2A antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). To inhibit MEK1, 25 µM PD98059 was added 1 h before curcumin treatment.

PI3K Activity Assay. PI3K activity was measured using PI3-Kinase enzyme-linked immunosorbent assay kit (Echelon Bioscience, Inc., Salt Lake City, UT) according to the manufacturer's instructions. This kit evaluates PI3K activity to detect the conversion of PI(4,5)P2 into PI(3,4,5)P3. In brief, cell culture and treatments were the same as described above. The cells were rinsed three times with ice-cold buffer A (137 mM NaCl, 20 mM Tris-HCl, pH 7.4, 1 mM CaCl₂, 1 mM MgCl₂, and 0.1 mM sodium orthovanadate) and harvested with ice-cold lysis buffer (buffer A plus 1% Nonidet P-40 and 1 mM phenylmethylsulfonyl fluoride). The cellular proteins were extracted by centrifugation. PI3K was isolated from equal amounts (100 µg) of the cellular protein by immunoprecipitation using anti-PI3K antibody (Upstate Biotechnology, Lake Placid, NY). The immunocomplexes bound onto protein A-agarose beads were incubated in the reaction buffer containing PI(4,5)P2 substrate and ATP, and the kinase reaction was stopped by pelleting the beads by centrifugation. The reaction mixtures were used for the following detection reaction. The absorbance of final solution was measured by a microplate reader at 450 nm. PI3K activity was calculated from the standard curve using various concentrations of PI(3,4,5)P3.

Electrophoretic Mobility Shift Assay for NF-B. NF-B activation was analyzed by electrophoretic mobility shift assay as described previously (Takada et al., 2004). Cells were incubated with or without 0.1 nM tumor necrosis factor (TNF-) for 30 min and then treated with 50 µM curcumin for 2 h. Eight micrograms of nuclear extracts was incubated with ³²P-end-labeled 45-mer double-stranded NF-B oligonucleotide from human immunodeficiency virus 1 long terminal repeat (5'-TTGTTACAAGGGACTTTCCGCTGGGGACTTTCCAGGGAGGCGTGG-3'; boldface sequence indicates NF-B binding site) for 15 min at 37°C, and the DNA-protein complex was resolved in a 6.6% native polyacrylamide gel. The radioactive bands from the dried gels were visualized and quantitated with a PhosphorImager with ImageQuant software (both from GE Healthcare).

NF-B p65 siRNA Transfection. SignalSilence NF-B p65 siRNA was purchased from Cell Signaling Technology. siCONTROL nontargeting siRNA purchased from Dharmacon (Chicago, IL) was used as a control siRNA. Cells were transfected with siRNA using Oligofectamine transfection reagent (Invitrogen) for 72 h according to the manufacturer's instructions. The final concentration of siRNA was 100 nM. To confirm the efficacy of NF-B p65 siRNA, Western blotting using anti-NF-B p65 antibody (Cell Signaling Technology) was performed as described above. The cytotoxic effect of NF-B p65 siRNA on U87-MG and U373-MG cells were determined using the WST-1 assay as described above.

Animal Studies. Adult nude mice (five mice per treatment group) were anesthetized with ketamine and xylazine as described previously (Ito et al., 2006). U87-MG cells (1.0 x 10⁶ cells in 20 µl of serum-free Dulbecco's modified Eagle's medium) were inoculated subcutaneously into the right flank of mice. Tumor growth was measured daily with calipers. Tumor volume was calculated as (L x W²)/2, where L is the length in millimeters, and W is the width in millimeters as described previously (Ito et al., 2006). When the tumors reached a mean volume of 50 to 70 mm³, a 10-µl intratumoral injection of curcumin (100 mg/kg/20 µl in DMSO/PBS) or the same dose of DMSO/PBS was given (day 0). Mice were euthanized by exposure to CO₂ 16 days after the initiation of treatment. Tumors were then removed, frozen rapidly, and used for Western blotting and immunohistochemical staining for LC3. All animal studies were performed in the veterinary facilities of The University of Texas M. D. Anderson Cancer Center in accordance with all institutional, state, and federal ethical regulations for experimental animal care.

Statistical Analysis. The data were expressed as means ± S.D. Statistical analysis was performed with the two-tailed Student's t test. The criterion for statistical significance was set at P < 0.05.

View larger version (28K): [in this window] [in a new window]   Fig. 1. Curcumin induces G2/M arrest, but not apoptosis, in U87-MG and U373-MG cells. A, the cytotoxic effect of curcumin as measured using the WST-1 assay. Cells were treated with different concentrations of curcumin for 72 h and subjected to the WST-1 cell proliferation assay. The viability of untreated cells was considered 100%. Data are the means of triplicate experiments; error bars, S.D. B, cell cycle analysis after treatment with curcumin. Cells were treated with 20 or 40 µM curcumin or 5 nM paclitaxel for 72 h and subjected to cell cycle analysis. Results shown are representative of three independent experiments. C, representative micrographs of the cells stained with TUNEL. Cells were treated with 40 µM curcumin or 5 nM paclitaxel for 72 h and subjected to TUNEL staining. Scale bars, 50 µm. D, quantitation of TUNEL-positive cells. Cells were treated as described above and subjected to TUNEL staining. TUNEL-positive cells were counted among 100 cells in an area, and more than three areas were selected at random. Data are the means of triplicate experiments; error bars, S.D. E, curcumin effect on clonogenic survival 14 days after the addition of curcumin. Clonogenic survival is expressed as a surviving fraction of nontreatment control for each treatment and is plotted on a semilogarithmic scale. Points, mean of triplicate experiments; bars, S.D.

	Results

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We then examined the effect of 20 or 40 µM curcumin on the cell cycle of U87-MG and U373-MG cells. Treatment with curcumin for 72 h induced G₂/M cell cycle arrest in a dose-dependent manner in both cell types (Fig. 1B). However, the percentage of the sub-G₁ population, which is indicative of apoptosis, did not increase much even after treatment with 40 µM curcumin: from 0.4 to 2.2% in U87-MG cells and from 0.3 to 3.7% in U373-MG cells. In contrast, treatment with 5 nM paclitaxel, which is known to induce apoptosis in malignant glioma cells (Kondo and Kondo, 2006), increased the percentage of the sub-G₁ population to 22.1% in U87-MG cells and to 15.7% in U373-MG cells.

To further examine whether curcumin induces apoptosis using an assay more specific for apoptosis, we performed TUNEL staining of U87-MG and U373-MG cells after treatment with curcumin. Treatment with 40 µM curcumin did not increase TUNEL-positive cells in either cell type (Fig. 1, C and D). In contrast, treatment with 5 nM paclitaxel for 72 h induced apoptosis in 17% of U87-MG cells and in 13% of U373-MG cells. These results indicate that curcumin induces the G₂/M arrest but not apoptosis in U87-MG and U373-MG cells.

To evaluate long-term efficacy of curcumin on cell survival, clonogenic assay was performed. U373-MG cells were treated with different concentrations of curcumin in six-well plates 24 h after seeding. Two weeks after treatment, colonies were counted. Curcumin suppressed the surviving fraction of U373-MG cells in a dose-dependent manner (Fig. 1E), indicating the cell killing effect of curcumin.

Curcumin Induces Autophagy in U87-MG and U373-MG Cells. An increasing number of studies have shown that cancer cells, including malignant glioma cells, undergo autophagy in response to various anticancer therapies (Ogier-Denis and Codogno, 2003; Gozuacik and Kimchi, 2004; Kondo et al., 2005). Thus, we examined whether curcumin induces autophagy in U87-MG and U373-MG cells. Electron microscopic analysis showed that autophagic vacuoles containing cellular material or membranous structures increased in U373-MG cells treated with 40 µM curcumin for 48 h compared with untreated control cells (Fig. 2A). To quantify the incidence of curcumin-induced autophagy, we performed the following assays. First, to quantify AVOs, which include autophagic vacuoles and lysosomes, we used acridine orange staining. Cells with AVOs showed enhanced red fluorescence that increased after treatment with curcumin in a dose-dependent manner (Fig. 2B). Moreover, addition of the autophagy inhibitor 3-MA inhibited the increase in the percentage of cells with enhanced AVOs after treatment with curcumin.

View larger version (26K): [in this window] [in a new window]   Fig. 2. Curcumin induces autophagy in U87-MG and U373-MG cells. A, representative electron micrographs of U373-MG cells treated with DMSO (control) or 40 µM curcumin for 48 h. Arrows, autophagic vacuoles; scale bar, 2 µm. B, quantification of cells with enhanced red fluorescence using flow cytometry after acridine orange staining. Cells were treated with DMSO (control) or the indicated concentrations of curcumin for 72 h and subjected to acridine orange staining. To inhibit autophagy, 2 mM 3-MA was added 24 h after the addition of curcumin. Results shown are representative of three independent experiments. C, representative micrographs of cells that show GFP-LC3 localization. Cells were transiently transfected with the GFP-LC3 plasmid for 24 h and treated with DMSO (control) or 40 µM curcumin for 72 h. D, Western blot analysis of LC3-I and LC3-II. Cells were treated with the indicated concentrations of curcumin for 48 h (top) or with 40 µM curcumin for the indicated times (bottom) and subjected to Western blotting. Anti--actin antibody was used as a loading control. Results shown are representative of two independent experiments. E, the effect of autophagy inhibition on the cytotoxicity of curcumin. After U87-MG and U373-MG cells were treated with 0, 20, or 40 µM curcumin with or without 2 mM 3-MA for 72 h, the cell viability was measured by WST-1 cell proliferation assay. The viability of untreated cells was considered 100%. Data are the means of triplicate experiments; error bars, S.D. *, P < 0.05 for curcumintreated cells with and without 3-MA.

In addition, we examined the expression of LC3-I and LC3-II using Western blot analysis, because LC3-II is closely associated with the membrane of autophagosomes (Kabeya et al., 2000). Expression of LC3-II increased in U87-MG and U373-MG cells treated with curcumin in dose- and time-dependent manners (Fig. 2D). Together, these results indicate that curcumin induces autophagy in U87-MG and U373-MG cells.

It is still debated whether cancer treatment-induced autophagy is a protective response or an anticancer effect (Kondo et al., 2005). To assess the role of curcumin-induced autophagy, we determined whether inhibition of autophagy by 3-MA affects the cytotoxicity of curcumin. When 3-MA inhibited curcumin-induced autophagy (Fig. 2B), the decreased viability of U87-MG and U373-MG cells treated with curcumin was reversed (P < 0.05). These results suggest that curcumin-induced autophagy is an antitumor effect and not a protective response to curcumin.

Curcumin Inhibits the Akt/mTOR/p70S6K Pathway and Activates the ERK Pathway in U87-MG and U373-MG Cells. Because the Akt/mTOR/p70S6K pathway is the main regulatory pathway that negatively regulates autophagy (Blommaart et al., 1995; Shigemitsu et al., 1999; Arico et al., 2001), we examined the effect of curcumin on it using Western blotting. Treatment with curcumin decreased phosphorylated Akt effectively for a period of 15 min to6hin both U87-MG and U373-MG cells (Fig. 3A), whereas the PI3K activity was not affected by curcumin (Fig. 3B). These results suggest that the upstream pathway of Akt was not influenced by curcumin treatment. Treatment with curcumin also decreased phosphorylated p70S6K gradually for 15 min to6hin U87-MG cells and for 1 to6hin U373-MG cells (Fig. 3A). Because the ERK pathway positively regulates autophagy in cancer cells on starvation (Ogier-Denis et al., 2000; Pattingre et al., 2003), we also examined this pathway after curcumin treatment. Treatment with curcumin increased phosphorylated ERK1/2 for 15 min to3hin U87-MG cells and for 15 min to6hin U373-MG cells (Fig. 3A). These results indicate that curcumin inhibited the Akt/mTOR/p70S6K pathway and activated the ERK pathway and suggest that both changes mediate curcumin-induced autophagy.

View larger version (31K): [in this window] [in a new window]   Fig. 3. Curcumin inhibits the Akt/mTOR/p70S6K pathway and activates the ERK pathway in U87-MG and U373-MG cells. A, Western blot analysis for the Akt/mTOR/p70S6K and ERK pathways. Cells were treated with 80 µM curcumin for the indicated times and subjected to Western blotting. Relative levels of phosphorylated Akt (phospho-Akt) to total Akt, those of phosphorylated p70S6K (phospho-p70S6K) to total p70S6K, and those of phosphorylated ERK1/2 (phospho-ERK1/2) to total ERK1/2 are indicated below the corresponding bands. The data shown are representative of three independent experiments. B, the change of PI3K activity by curcumin. PI3K activity was determined by measuring the conversion of PI(4,5)P2 into PI(3,4,5)P3 using enzyme-linked immunosorbent assay kit. U87-MG and U373-MG cells were treated with 80 µM curcumin for the indicated time (minutes). Data are the means of triplicate experiments; error bars, S.D. C, Western blot analysis of protein phosphatase phosphorylation. U87-MG and U373-MG cells were treated with 80 µM curcumin for up to 3 h and then subjected to Western blotting for detection of phosphorylated PP1 and PP2A and total PP1 and PP2A. Relative levels of phosphorylated PP1 (phospho-PP1) to total PP1 and those of phosphorylated PP2A (phospho-PP2A) to total PP2A are indicated below the corresponding bands. The data shown are representative of three independent experiments.

Activation of the Akt Pathway Inhibits Curcumin-Induced Autophagy and Cytotoxicity in U87-MG and U373-MG Cells. We and others have shown that the Akt/mTOR/p70S6K pathway mediates autophagy induced by some anticancer therapies (Blommaart et al., 1995; Shigemitsu et al., 1999; Takeuchi et al., 2005). Thus, we used rAkt1 to activate the Akt pathway as described previously (Takeuchi et al., 2004) so we could examine the role of this pathway in curcumin-induced autophagy. U87-MG cells were treated with 80 µM curcumin, 500 ng/ml rAkt1, or both for 3 h for Western blotting. The addition of rAkt1 inhibited the curcumin-induced decrease in phosphorylated Akt and phosphorylated p70S6K in U87-MG cells (Fig. 4A); we obtained similar results with U373-MG cells (data not shown). The addition of rAkt1 significantly decreased curcumin-induced autophagy in both cell types (P < 0.05; Fig. 4B). Furthermore, the addition of rAkt1 significantly inhibited curcumin-induced cytotoxicity in both cells (P < 0.05; Fig. 4C). These results indicate that curcumin-induced inactivation of the Akt/mTOR/p70S6K pathway plays a role in the induction of autophagy and suggest that the autophagy negatively regulated by this pathway may be associated with cell death.

View larger version (30K): [in this window] [in a new window]   Fig. 4. Activation of the Akt pathway by rAkt inhibits curcumin-induced autophagy and cytotoxicity in U87-MG and U373-MG cells. A, Western blot analysis of the Akt/p70S6K pathway. U87-MG cells were treated with 80 µM curcumin, 500 ng/ml rAkt1, or both for 3 h and subjected to Western blotting. Relative levels of phosphorylated Akt (phospho-Akt) to total Akt and those of phosphorylated p70S6K (phospho-p70S6K) to total p70S6K are indicated below the corresponding bands. The data shown are representative of two independent experiments. B, quantification of the cells with GFP-LC3 dots. Cells were transfected with the GFP-LC3 plasmid for 24 h and then treated with 40 µM curcumin, 500 ng/ml rAkt1, or both for 72 h, and the percentage of cells with GFP-LC3 dots among the total number of cells with GFP expression was determined. Data are the means of triplicate experiments; error bars, S.D.; **, P < 0.01. C, cytotoxic effect measured using the WST-1 assay. Cells were treated with different concentrations of curcumin, 500 ng/ml rAkt1, or both for 72 h and subjected to the WST-1 cell proliferation assay. The viability of untreated cells was considered 100%. Data are the means of triplicate experiments; error bars, S.D.; *, P < 0.05.

View larger version (30K): [in this window] [in a new window]   Fig. 5. Inhibition of the ERK pathway by PD98059 (PD) inhibits curcumin-induced autophagy and induces apoptosis in U87-MG and U373-MG cells. A, Western blot analysis of the ERK pathway and LC3. U373-MG cells were pretreated with the indicated concentrations of PD98059 for 1 h, treated with 80 µM curcumin for 1 h, and then subjected to Western blotting for detection of phosphorylated ERK1/2 (phospho-ERK1/2) and total ERK1/2. For the detection of LC3, cells were pretreated with PD98059 for 1 h and then treated with 40 µM curcumin for 72 h. B, quantitation of cells with GFP-LC3 dots among cells treated with 40 µM curcumin only or 40 µM curcumin and 25 µM PD98059. Cells were transiently transfected with the GFP-LC3 plasmid for 24 h and then treated as described for 72 h. The number of cells with GFP-LC3 dots was counted, and their percentage among the total number of cells expressing GFP was determined. Data are the mean of triplicate experiments; error bars, S.D.; **, P < 0.01; *, P < 0.05. C, Western blot analysis for PARP cleavage. U373-MG cells were treated with curcumin, PD98059, or both as described above and subjected to Western blotting 72 h later. Anti--actin antibody was used as a loading control. Results shown are representative of two independent experiments. D, cytotoxic effect measured using the WST-1 assay. Cells were treated with 40 µM curcumin only or 40 µM curcumin and 25 µM PD98059 and subjected to the WST-1 assay 72 h later. Data are the means of triplicate experiments; error bars, S.D.; **, P < 0.01.

We next determined the extent of the inhibitory effect of PD98059 on curcumin-induced autophagy in U87-MG and U373-MG cells by examining GFP-LC3 localization. The percentage of cells with GFP-LC3 dots decreased significantly in U87-MG and U373-MG cells treated with PD98059 and curcumin compared with that in the cells treated with curcumin alone (P < 0.05; Fig. 5B). The ERK pathway is known as an antiapoptosis pathway (Xia et al., 1995), although prolonged activation of the ERK pathway has been shown to induce apoptosis (Lee et al., 2003; Cagnal et al., 2006). Then we examined whether its inhibition induces apoptosis in curcumin-treated tumor cells. PARP was clearly cleaved in U373-MG cells treated with both PD98059 and curcumin, whereas it was not in the untreated cells or in those treated with PD98059 or curcumin alone (Fig. 5C). Similar results were obtained using U87-MG cells (data not shown). It is interesting that curcumin-induced cytotoxicity was significantly enhanced in the cells treated with PD98059 (P < 0.05 in both cell types; Fig. 5D).

These results indicate that blocking the ERK pathway has a negative effect on the curcumin effect and therefore suggest that the ERK pathway is involved in curcumin-induced autophagy in U87-MG and U373-MG cells. This pathway protects these cells from apoptosis, at least to some extent, although we need more direct evidence.

NF-B Inhibition Is Not Involved in the Cytotoxicity of Curcumin in U87-MG and U373-MG Cells. Many studies showed that curcumin inhibits NF-B activity and induces apoptosis in various cancer cell types (Bharti et al., 2003; Aggarwal et al., 2004, 2006). Thus, we examined whether inhibition of NF-B was involved in the anticancer effect of curcumin in U87-MG and U373-MG cells. First, we examined active NF-B using electrophoretic mobility shift assay in cells with no treatment and cells treated with curcumin, TNF, or both. We also used KBM-5 leukemia cells as a positive control for TNF-activated NF-B (Takada et al., 2004). Both U87-MG and U373-MG cells showed very little or no active NF-B (Supplementary Fig. S1A). When cells were treated with 0.1 nM TNF for 30 min, U373-MG and U87-MG cells showed, respectively, moderate and modest levels of active NF-B, whereas KBM-5 cells showed a very high level of active NF-B (Supplementary Fig. S1A). Treatment with 50 µM curcumin for 2 h inhibited TNF-induced active NF-B by 50% in U373-MG cells only.

View larger version (67K): [in this window] [in a new window]   Fig. 6. Curcumin inhibits the growth of malignant glioma cells in vivo by inducing autophagy. A, tumor growth on day 16 after the initiation of curcumin treatment. U87-MG cells (1 x 106) were inoculated subcutaneously into the nude mice. When the tumors reached 50 to 70 mm3 in volume, intratumoral injections of curcumin (100 mg/kg in DMSO/PBS) or DMSO/PBS (control) were administered every 24 h for 7 days. B, Western blot analysis of excised tumors for LC3. Tumors were removed on day 16, and the proteins were isolated and subjected to Western blotting. Anti--actin antibody was used as a loading control. Results shown are representative of two independent experiments. C, representative micrographs of immunohistochemical staining using anti-LC3 antibody. Tumor-bearing animals were treated, and the tumors were removed as described above. Tumor samples were snap-frozen, sliced to 10-µm thickness, and subjected to immunohistochemical staining. Scale bars, 50 µm.

Curcumin Inhibits Growth of Subcutaneous Tumors by Inducing Autophagy. We determined whether curcumin inhibits growth and induces autophagy in malignant glioma in vivo. Nude mice were inoculated subcutaneously with 1 x 10⁶ U87-MG cells. When tumors reached 50 to 70 mm³ in volume, intratumoral injections of curcumin (100 mg/kg in DMSO in PBS) or vehicle (DMSO in PBS) were administered every 24 h for 7 days, and tumor growth was observed until 16 days after the initiation of treatment. On day 16, tumor growth was inhibited significantly in tumors treated with curcumin compared with the control-treated tumors (3.5 ± 2.8-fold versus 12.5 ± 5.9-fold; P < 0.05) (Fig. 6A). To examine whether curcumin induces autophagy in vivo, we examined the expression of LC3, especially LC3-II, using Western blotting and immunohistochemical staining. The expression of LC3-II increased remarkably in tumors treated with curcumin compared with that in the control-treated tumors (Fig. 6B). The expression of total LC3 in the cytoplasm detected by immunohistochemical staining using an anti-LC3 antibody also increased in the tumors treated with curcumin compared with the control-treated tumors (Fig. 6C). These results, together with the in vitro findings with 3-MA (Fig. 2E), suggest that curcumin inhibits tumor growth in vivo by inducing autophagy.

	Discussion

Top Abstract Materials and Methods Results Discussion References

 
The results of this study showed that curcumin induced G₂/M cell cycle arrest and autophagy, but not apoptosis, in U87-MG and U373-MG cells. Regarding signal pathways, curcumin inhibited the Akt/mTOR/p70S6K pathway and activated the ERK pathway, resulting in autophagy. The autophagy regulated by these two different pathways differently influenced the cytotoxicity of curcumin. Furthermore, curcumin effectively inhibited tumor growth and induced autophagy in the xenograft tumor model of U87-MG cells. These results demonstrate that curcumin may be a promising agent for the treatment of patients with malignant glioma. To the best of our knowledge, this study is the first to demonstrate that curcumin induces autophagy in cancer cells in vitro and in vivo.

This study clearly demonstrated that curcumin inhibits the Akt/mTOR/p70S6K pathway and activates ERK signaling, resulting in the induction of autophagy (Figs. 2 and 3). Several other studies have also shown that curcumin inhibits the Akt/mTOR/p70S6K pathway in various cancer cells including leukemia, renal cancer, breast cancer, and prostate cancer cells (Woo et al., 2003; Bava et al., 2005; Aggarwal et al., 2006; Beevers et al., 2006). Some investigators reported the effect of curcumin on ERK signaling but with different results (Squires et al., 2003). Woo et al. (2005) showed that curcumin repressed the phorbol ester-induced activation of ERK, whereas Collett and Campbell (2004) found no effect of curcumin on ERK. Because curcumin modulates many pathways (Shishodia et al., 2005; Aggarwal et al., 2006), its detailed mechanisms may vary depending on the cancer cell type. In the context of induction of autophagy, Ellington et al. (2006) showed that the natural products triterpenoid B-group soyasaponins induced autophagy by inhibiting Akt signaling and enhancing ERK activity, in accord with our findings. This combination of Akt inhibition and ERK activation may be one of the common mechanisms of autophagy induction by anticancer agents.

Because the extent of autophagy increased in dose- and time-dependent manners, we concluded that autophagy is a response of U87-MG and U373-MG cells to curcumin. Our results clearly indicated that the cytotoxic effect of curcumin on these cells is caused by autophagy but not by apoptosis. The overall effect of curcumin is as an anticancer agent both in vitro and in vivo, as shown in other cancer cells (Shishodia et al., 2005; Aggarwal et al., 2006). Although autophagy is designated programmed cell death type II, whether autophagy actually leads cells to death or protects them from death has been a controversial issue (Gozuacik and Kimchi, 2004; Takada et al., 2004). Some investigators knocked down autophagy-related (Atg) genes using siRNA and specifically inhibited autophagy but reached opposite conclusions depending on their experimental system. For example, in an apoptosis-defective system in which Bax and Bak were both knocked out (i.e., Bax/Bak^-/-), siRNA for Beclin 1 or Atg5 inhibited etoposide-induced autophagy and led cells to survival, whereas siRNA for Atg5 or Atg7 inhibited autophagy caused by interleukin-3 deprivation and killed more cells (Shimizu et al., 2004; Lum et al., 2005). One possibility is that autophagy kills or protects cells depending on how autophagy is induced. That is, interleukin-3 deprivation-induced autophagy is supposed to be a survival mechanism, so inhibition of this autophagy leads to death; etoposide induces cell death, so inhibition of this autophagy saves cells from death. In this study, we examined the role of autophagy by manipulating the regulatory pathways individually, because the Akt and ERK pathways are known to regulate autophagy, but with opposite effects: the Akt pathway regulates autophagy negatively, whereas the ERK pathway regulates it positively. Activation of the Akt pathway using rAkt1-inhibited curcumin-induced autophagy and cytotoxicity (Fig. 4). On the other hand, inhibition of the ERK pathway using PD98059 inhibited autophagy and induced apoptosis, thus enhancing cytotoxicity (Fig. 5). These results imply that the role of autophagy on cell death is pathway-specific. That is, the autophagy the Akt pathway inhibits confers cell death, and the autophagy the ERK pathway induces confers cell survival. This hypothesis can explain the double effect autophagy has on cell death, and it is worth being evaluated in different experimental systems.

NF-B is one of the main targets of curcumin for its anticancer effect (Singh and Aggarwal, 1995; Bharti et al., 2003; Aggarwal et al., 2004). However, we found that U87-MG and U373-MG cells had very little or no constitutively active NF-B. Furthermore, when we completely knocked down NF-B p65, the viability of these cell types did not change (Supplementary Fig. S1). These results indicate that the anticancer effect of curcumin and the autophagy we detected in these cell types are not caused by inhibition of NF-B. However, curcumin can be used to inhibit active NF-B that is induced by chemokines or other anticancer treatments, as shown in leukemia and cervical cancer cells (Xia et al., 1995; Bava et al., 2005). For example, radiation induces NF-B activity in malignant glioma cells, which implies a resistant mechanism (Raju et al., 1997). Thus, curcumin may need to be used in combination with radiation or other chemotherapeutic agents for treating malignant glioma to demonstrate its inhibitory effect of NF-B.

Our results showed that curcumin significantly inhibited the growth of malignant glioma both in vitro and in vivo. An increasing number of studies have shown the anticancer efficacy of curcumin in preclinical and clinical settings. In subcutaneous animal models of various cancer cell types, curcumin effectively inhibited the growth of tumors (Shishodia et al., 2005; Aggarwal et al., 2006). Furthermore, a recent study reported that curcumin suppressed lung metastasis of breast cancer cells when used as a single agent or in combination with paclitaxel (Aggarwal et al., 2005). Several phase I clinical studies have demonstrated that curcumin was well tolerated up to 12 g/day without major adverse effects (Sharma et al., 2001, 2004; Lao et al., 2006). A phase II clinical trial for patients with pancreatic cancer is ongoing at our institute. However, absorption and bioavailability of curcumin outside the colon is very problematic. Therefore, the intratumoral injection of rather large concentrations of curcumin that we used in this study might be not very useful clinically for human gliomas. Convection-enhanced delivery has been developed as a new technique of direct injection to increase drug uptake and distribution to large regions of the brain tumor by applying a pressure gradient (Lopez et al., 2006). With this method, curcumin can be delivered to malignant gliomas directly and efficiently while limiting toxicity to surrounding normal tissues.

In summary, we have shown for the first time that curcumin induces autophagy in malignant glioma cells both in vitro and in vivo. The Akt/mTOR/p70S6K and ERK1/2 pathways are involved in curcumin-induced autophagy. Our results suggest that effect of autophagy on cell death may be dependent on its regulatory pathways. We recommend that the use of curcumin as a new anticancer agent for malignant glioma should be pursued further because of its prominent effect and its new anticancer mechanism of inducing autophagy.

	Acknowledgements

 
We thank Dr. Noboru Mizushima for the generous gift of the GFP-LC3 plasmid, E. Faith Hollingsworth for technical help, and Karen Philips for editing the manuscript. We also thank the High Resolution Electron Microscopy Facility for assistance with the electron microscopy.

	Footnotes

 
Institutional Research Grant from The University of Texas M. D. Anderson Cancer Center (to Y.K.), United States Public Health Service grants CA088936 and CA108558 (to S.K.), Institutional Cancer Core grant CA16672, and a generous donation from the Anthony D. Bullock III Foundation (to S.K. and Y.K.).

ABBREVIATIONS: NF-B, nuclear factor B; mTOR, mammalian target of rapamycin; ERK1/2, extracellular signal-regulated kinases 1 and 2; p70S6K, p70 ribosomal protein S6 kinase; PTEN, phosphatase and tensin homolog; MEK1, mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1; DMSO, dimethyl sulfoxide; 3-MA, 3-methyladenine; PI3K, phosphatidylinositol 3-phosphate kinase; PD98059, 2'-amino-3'-methoxyflavone; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AVO, acidic vesicular organelle; GFP, green fluorescent protein; LC3, light chain 3; PARP, poly(ADP-ribose) polymerase; TNF, tumor necrosis factor; PBS, phosphate-buffered saline; siRNA, small interfering RNA; PP1, protein phosphatase type 1; PP2A, protein phosphatase type 2A; Atg, autophagy-related gene.

The online version of this article (available at http://molpharm.aspetjournals.org) contains supplemental material.

Address correspondence to: Dr. Seiji Kondo, Department of Neurosurgery/BSRB1004, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030. E-mail: seikondo{at}mdanderson.org

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